RNA-seq analysis
From raw FASTQ files to differential expression, using a pinned, reproducible pipeline.
What you receive
- Quality control: FastQC and MultiQC report
- Alignment and quantification (STAR + Salmon)
- Gene-level count and TPM matrices
- Differential expression (DESeq2) for your chosen comparisons
- PCA, sample-distance and volcano plots
- Methods text and complete software versions
What we need from you
- Raw FASTQ files (single-end or paired-end, gzip-compressed)
- Organism and reference genome (for example human or mouse)
- Sample names, conditions and replicates
- The comparisons you want tested